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迪亚诺娃/齐格F（ab&8217；）2抗人IgG（Fc）-RPE，MinX Bo，Ho，Ms/1 ml/109-116-098
| Artikelnummer | 109-116-098 |
|---|---|
| Wirtsspezies | Ziege |
| IgG-Form | IgG F(ab')2-Fragment |
| Spezies-Reaktivität | Human |
| Spezifität | IgG (Fc-Fragment) |
| Isotyp | Ig |
| Klonalität (Mono-/Polyklonal) | polyklonal |
| Anwendung | Durchflusszytometrie (Flow Cytometry), Immuncytochemie, Immunfluoreszenz |
| Konjugation | R-Phycoerythrin (R-PE) |
| Maximale Anregung | 490 nm, 545 nm, 566 nm |
| Maximale Emission | 580 nm |
| Keine Kreuzreaktivität (MinX) mit | Maus, Pferd, Rind |
| Verdünnung | für die meisten Anwendungen 1:50 – 1:200 |
| Format | 0,05% NaN3, 15 mg/ml BSA (IgG- und Protease-frei), 250 mM NaCl, affinitätsgereinigt durch antigenspezifische Affinitätschromatographie, in 10 mM PBS (pH 7,6), Lyophilisat |
| Anwendungshinweis | IgG, Fc gamma-spezifisch |
| Zweckbestimmung | nur für Forschungszwecke |
| Produktlinie / Thema | Jackson ImmunoResearch |
| Hersteller / Marke | Jackson ImmunoResearch |
- 109-116-098 – Datenblatt
Conjugate
Phycoerythrin (R-PE) is among several kinds of light-harvesting phycobiliproteins found in red, blue-green, and cryptomonad algae. We offer R-PE, the form found in red macrophytic algae (seaweed). After phycobiliproteins are conjugated to secondary antibodies, there is little fluorescence quenching, which results in conjugates of high specific fluorescence compared with conventional fluorophore-antibody conjugates. R-PE can be excited by light over a wide range of the visible spectrum is highly water soluble, has a relatively low isoelectric point, and lacks potentially sticky carbohydrates.
It should be noted that the relatively high molecular weight of R-PE may preclude its use in procedures requiring good penetration into cells and tissues. It is predominantly intended for surface labeling of cells for flow cytometry.
