| Artikelnummer | 115-175-146 |
|---|---|
| Wirtsspezies | Ziege |
| IgG-Form | IgG Gesamtmolekül |
| Spezies-Reaktivität | Maus |
| Spezifität | IgG (H+L) |
| Isotyp | Ig |
| Klonalität (Mono-/Polyklonal) | polyklonal |
| Anwendung | Durchflusszytometrie (Flow Cytometry), ELISA (Enzyme Linked Immunosorbent Assay), Immuncytochemie, Immunfluoreszenz, Immunhistochemie (Gefrierschnitte), Immunhistochemie (IHC), Immunhistochemie (Paraffingewebe) |
| Konjugation | Cy5 (Indodicarbocyanin) |
| Maximale Anregung | 650 nm |
| Maximale Emission | 670 nm |
| Keine Kreuzreaktivität (MinX) mit | Human, Kaninchen, Pferd, Rind, Schwein |
| Verdünnung | für die meisten Anwendungen 1:100 – 1:400 |
| Format | 0,05% NaN3, 15 mg/ml BSA (IgG- und Protease-frei), 250 mM NaCl, affinitätsgereinigt durch antigenspezifische Affinitätschromatographie, in 10 mM PBS (pH 7,6), Lyophilisat |
| Zweckbestimmung | nur für Forschungszwecke |
| Produktlinie / Thema | Jackson ImmunoResearch |
| Hersteller / Marke | Jackson ImmunoResearch |
- 115-175-146 – Datenblatt
Conjugate
Cy5 conjugates are excited maximally at 650 nm and fluoresce maximally at 670 nm. They can be excited to about 98% of maximum with a krypton/argon laser (647 nm line) or to about 63% of maximum with a helium/neon laser (633 nm line). Cy5 can be used with a variety of other fluorophores for multiple labeling due to a wide separation of its emission from that of shorter wavelength-emitting fluorophores. However, we recommend Alexa Fluor 647 as the preferred far-red-fluorescing dye because it is brighter than Cy5 in aqueous mounting media. The reverse is true when mounting in non-polar, plastic mounting media, i.e. Cy5 is brighter than other far-red-fluorescing dyes, including Alexa Fluor 647 and DyLight 650.
